Study of the Qualitative and Semi-quantitative Analysis of Grape Seed Extract by HPLC

Abstract

The main aim of this study is to carry out a qualitative and semiquantitativeanalysis of tannin extracts as an alternative to theofficial analysis method ISO 14088 – IUC 32, so that a correlationbetween the two methods is established.From the point of view of the chemical composition, tannins areclassified into two major groups:i) condensed tannins, also called flavanols or catechins, and ii)hydrolysable tannins, also called pyrogallic tannins.Today, the most widely used conventional extracts are quebracho,mimosa, chestnut, and tara. Quebracho and mimosa are condensedtannins, whereas chestnut and tara are hydrolysable tannins.The following extracts were used in this study: tara powder,commercial mimosa and quebracho extracts and extractsderived from grape seed, containing both condensed andhydrolysable extracts.The development of this new method will allow a faster andless expensive estimate of the amount of tannins present in atannin extract.