Kinetics of Inhibition of Type I Collagenase by Dialdehyde Cellulose in Stabilization of Type I Collagen

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G. Jayakumar
N. Usharani
A. Yasothai
S. Kanth
J. Raghava Rao


Collagen is one of the widely studied biomaterial for various industrial applications. However, search of eco-friendly and biocompatible stabilizing agent is a thrust research domain. In this research work, application of dialdehyde cellulose (DAC)was studied to understand the effect on the enzymatic and conformational stability in collagen. The secondary structure of collagen is not significantly altered on interaction with DAC. But, it was found that DAC lead to changes in the amplitude of the circular dichroic (CD) spectrum but did not alter the triple helical conformation of collagen. DAC treated collagen exhibited 93% resistance to collagenolytic hydrolysis. Conversely, DAC treated collagenase exhibited 89% inhibition against collagen degradation and the inhibition was found tobe concentration dependent. The kinetics of inhibition of collagenase by DAC was derived from the extent of hydrolysisof (2-furanacryloyl-L-leucyl-glycyl-L-prolyl-L alanine), FALGPA. DAC exhibited non-competitive mode of inhibition against collagenase. CD data on DAC-modified collagenase substantiate the hypothesis that the inhibition of collagenase by DAC arises from secondary and quaternary structural changes in the enzyme. Gaining new insights in understanding the mechanism of stabilization of collagen by DAC through kinetics of inhibition of collagenase was presented.

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