Antioxidation Activity of Cysteine and Cystine Prepared from Sheep Wool

Main Article Content

Ján Matyašovský
Ján Sedliačik
Peter Šimon
Igor Novák
Peter Jurkovič
Peter Duchovič
Mariana Sedliačiková
Zuzana Cibulková
Matej Mičušík
Angela Kleinová


Amino acid cysteine and its oxidized dimer form,?cystine, can be found in hair, nails, hoofs, feather, wool, etc. The main focus of this research was the preparation of cysteine and cystine from wool and testing of its antioxidation properties in the reduced and oxidized forms. Preparation technology was optimized on a laboratory scale and the samples were characterized by FTIR-ATR spectroscopy and X-ray photoelectron spectroscopy (XPS). Antioxidation activity of cysteine and cystine was studied in a?polyethylene glycol (PEG) matrix employing the non-isothermal differential scanning calorimetry (DSC). In the data treatment, the Berthelot-Hood equation was chosen as the temperature function. The kinetic parameters enabled the assessment of the induction period of oxidation for application temperatures. For the comparison of the stabilizing effect, the protection factors and antioxidant efficiency were calculated. The results show that the antioxidant activity of the samples depends on the preparation procedure, purity, etc. Protection factors decreased with increasing temperature and decreasing concentration. 

In the second part of the study, cysteine was tested in its reduced and oxidized form for the model polycondensation system of the urea-formaldehyde formulations. The quality parameters of these adhesive mixtures were verified on wood-based panels to determine formaldehyde emission and physical and mechanical properties. The results showed ca. 44% decrease of formaldehyde emission and increase of the shear strength of glued joint for all modifications in comparison with?the reference sample. 

Article Details