Preparation and Characterization of Decellularized ECM Hydrogel from Adipose Tissues of Hide/Skin Unlocking Untapped Resource Potential

Abstract

Raw fleshing wastes is the major solid waste generated during leather manufacturing. Utilization of the fleshing waste for the development of biomaterial holds a promising and sustainable solution for leather manufacturing. Decellularized hydrogels possess the ability to differentiate into specific tissue types of the host microenvironment and supports the cell adhesion, migration, proliferation, and differentiation of cells. Hydrogel is a biocompatible biomaterial that has the potential to mimic extracellular matrix (ECM). ECM-based biomaterials derived from animal tissues are ideal for applications in tissue engineering. In the present research work, dECM-derived hydrogels from the decellularized raw fleshing waste was prepared and characterized for its possible applications in tissue engineering. The fleshing waste was decellularized using varying concentrations of SDS and Triton-X 100 detergents. The tissue sample treated with 0.1% SDS and 1% Triton-X 100 for decellularization exhibited higher collagen and glycosaminoglycans (GAG) retention. The study also provides a possible way for the transformation of tannery fleshing waste into high value products.