Current Understanding of the Role of Surface Charge in the Mass Transfer of Enzymes into Animal Hide/Skin A Comprehensive Analysis and Review

Abstract

The key point for enzymes to effectively play their roles in the enzymatic animal hide/skin collagen purification processing is to pass through the diffusion barriers and evenly disperse in the hide/skin. Enzyme and collagen proteins can dissociate and adsorb H+ to generate surface charges at the solid-liquid interface of animal hides/skins. The surface charge interaction between the enzyme and the hide/skin is an important factor affecting the mass transfer of enzymes into animal hides/skins. In this review, the structure, composition and charge characteristics of the permeation channels, the generation mechanism of surface charge at the solid-liquid interface, and the factors that affect the surface charge between enzymes and permeation channels were summarized. Especially, the surface charge interaction between the enzymes and the micro-nano permeation channels was first highlighted. In addition, the application of charge regulation methods to enhance the permeability of enzymes into the animal hide/skin was introduced, and new ideas were put forward to reduce the resistance of solid-liquid interface charge for achieving rapid mass transfer and uniform diffusion of the enzymes. This review has great guiding significance for enzymatic animal hide/skin collagen purification and the preparation of collagen-based raw materials.