The effect of neuromast position on bone fragmentation in the third suborbital bone of the cave-dwelling populations of Astyanax mexicanus

Abstract

By Rachel Rowland, Biological Sciences; Tess Palluzzi, Biology of Animals

Advisor: Joshua Gross

Presentation ID: AM_ATRIUM21

Abstract: Many structural differences can be observed pertaining to the craniofacial development between the cave- and surface-dwelling forms of Astyanax mexicanus. A variety of bone fragmentation occurs in the craniofacial region. Based on these differences in development, specifically in the third suborbital bone (SO3), independent carving events have been observed. After imaging and following these events, we sought to determine a correlation between individual carving events and the position of neuromasts on the third suborbital bone. With the utilization of two staining techniques, Calcein and Daspei staining, we are able to get a more detailed look at the third suborbital bone under fluorescent light. On a weekly basis, we analyzed images of both surface and cave-dwelling individuals in order to determine if carving deepend throughout time and development. Ultimately, the goal was to gain insight on neuromast correlation in comparison to bone fragmentation throughout various stages of life of Astyanax mexicanus.